Journal:

Article Title: The erm (T) Gene Is Flanked by IS 1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus

doi: 10.1128/AAC.49.10.4347-4350.2005

Figure Lengend Snippet: Genetic organization of erm(T) resistance element and flanking regions in S. gallolyticus subsp. pasteurianus NTUH-7421. (A) Southern blot hybridization of erm(T) probe to S. gallolyticus subsp. pasteurianus NTUH-7421 genomic DNA after digestion with restriction enzymes ClaI, DdeI, HaeIII, HindII, and EcoRI (lanes 1 to 5, respectively). Lane M, DNA marker (digoxigenin-labeled DNA Molecular Weight Marker II′ [Roche]). (B) Genetic organization of erm(T) resistance element and flanking regions in S. gallolyticus subsp. pasteurianus NTUH-7421. Arrows represent putative open reading frames. The restriction sites are also shown.

Article Snippet: One erythromycin-susceptible reference strain (ATCC 43144) of S. gallolyticus subsp. pasteurianus and two isolates of erm (T)-negative S. gallolyticus subsp. pasteurianus [one was erythromycin resistant due to the presence of an erm (B) gene, and the other was erythromycin susceptible] were used as negative controls.

Techniques: Southern Blot, Hybridization, Marker, Labeling, Molecular Weight

Journal:

Article Title: The erm (T) Gene Is Flanked by IS 1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus

doi: 10.1128/AAC.49.10.4347-4350.2005

Figure Lengend Snippet: Southern blot hybridization of erm(T) and IS1216V probe to S. gallolyticus subsp. pasteurianus strains. (A) Hybridization with an erm(T)-specific probe. Lanes 1 to 6 show erm(T)-positive isolates. Lane 1, NTUH-7421; lane 2, NTUH-8819; lane 3, NTUH-7499; lane 4, NTUH-3004; lane 5, NTUH-1043; lane 6, NTUH-4807; lanes 7 and 8, erm(T)-negative clinical isolates NTUH-1443 and NTUH-4046, respectively; lane 9, S. gallolyticus subsp. pasteurianus ATCC 43144; lane M, DNA marker (digoxigenin-labeled DNA Molecular Weight Marker II′ [Roche]). (B) Hybridization with IS1216V-specific probe. Lanes 1 to 6 and 8 to 13, erm(T)-positive isolates, as in lanes 1 to 6 in panel A. Lanes 7 and 14, erm(T)-negative clinical isolate NTUH-1443.

Article Snippet: One erythromycin-susceptible reference strain (ATCC 43144) of S. gallolyticus subsp. pasteurianus and two isolates of erm (T)-negative S. gallolyticus subsp. pasteurianus [one was erythromycin resistant due to the presence of an erm (B) gene, and the other was erythromycin susceptible] were used as negative controls.

Techniques: Southern Blot, Hybridization, Marker, Labeling, Molecular Weight

Journal:

Article Title: The erm (T) Gene Is Flanked by IS 1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus

doi: 10.1128/AAC.49.10.4347-4350.2005

Figure Lengend Snippet: Localization of erm(T) on I-CeuI-generated chromosome fragments of S. gallolyticus subsp. pasteurianus isolates. (A) I-CeuI fragment restriction patterns separated by pulsed-field gel electrophoresis. (B) Hybridization with a probe specific for the erm(T) gene. (C) Hybridization with a 16S rRNA gene-specific probe. Lane M, molecular size standard (Saccharomyces cerevisiae chromosomal DNA). Lanes 1 to 6 show erm(T)-positive isolates. Lane 1, NTUH-7421; lane 2, NTUH-8819; lane 3, NTUH-7499; lane 4, NTUH-3004; lane 5, NTUH-1043; lane 6, NTUH-4807; lanes 7 and 8, erm(T)-negative clinical isolates NTUH-1443 and NTUH-4046, respectively.

Article Snippet: One erythromycin-susceptible reference strain (ATCC 43144) of S. gallolyticus subsp. pasteurianus and two isolates of erm (T)-negative S. gallolyticus subsp. pasteurianus [one was erythromycin resistant due to the presence of an erm (B) gene, and the other was erythromycin susceptible] were used as negative controls.

Techniques: Generated, Pulsed-Field Gel, Electrophoresis, Hybridization

Journal:

Article Title: The erm (T) Gene Is Flanked by IS 1216V in Inducible Erythromycin-Resistant Streptococcus gallolyticus subsp. pasteurianus

doi: 10.1128/AAC.49.10.4347-4350.2005

Figure Lengend Snippet: Alignment of the leader peptide-encoding sequences of erm(T) in S. gallolyticus subsp. pasteurianus NTUH-7421, erm(GT) in Lactobacillus species (GenBank accession number M64090), and staphylococcal erm(C) (GenBank accession number M17990). A dash indicates an identical nucleotide and a dot indicates a gap. TAA (stop codon) is underlined.

Article Snippet: One erythromycin-susceptible reference strain (ATCC 43144) of S. gallolyticus subsp. pasteurianus and two isolates of erm (T)-negative S. gallolyticus subsp. pasteurianus [one was erythromycin resistant due to the presence of an erm (B) gene, and the other was erythromycin susceptible] were used as negative controls.

Techniques:

Journal: Journal of molecular biology

Article Title: Conformational Change of the Hairpin-like-structured Robo2 Ectodomain Allows NELL1/2 Binding.

doi: 10.1016/j.jmb.2022.167777

Figure Lengend Snippet: Figure 4. FRET-based indicators to assess the topological conformation of the Robo2 ectodomain. (A) Schematic diagrams of the intramolecular FRET constructs. Gamillus/GFP and mTurquoise2/CFP were fused to the N- and C-termini of the Robo2 ectodomain, respectively. A deletion construct of both the Ig1 and FNIII3 domains was prepared to decrease the distance between Gamillus and mTurquoise2. (B) Schematic overview of the FRET experiments. If Gamillus and mTurquoise2 are in close proximity due to conformational change of the Robo2 ectodomain, FRET (emission of 515-nm light after excitation with 430-nm light) may occur. (C) Emission scans of Robo2-ECD and Robo2-ECD/DIg1&FNIII3 constructs at pH 7.0. The excitation wavelength was 430 nm and emission was measured at 460–560 nm. (D) The FRET ratio was defined as the ratio of emission intensity of Gamillus to the emission intensity of mTurquoise2 (515 ± 5 nm/475 ± 5 nm). Each value represents the mean ± SD of at least triplicate results. *, p < 0.05 (Student’s t-test).

Article Snippet: Gamillus/ pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 124837).38 pLifeAct- mTurquoise2 was a gift from Dorus Gadella (Addgene plasmid # 36201).37 Deletion mutants lacking the coding sequence for both the Ig1 domain (residues 43–144) and the FNIII3 domain (residues 740–826) or only Ig1 domain were generated by inverse PCR and designated as Gam:Rob o2-ECD/DIg1&FNIII3:mTq2 and Gam:Robo2ECD/DIg1:mTq2, respectively.

Techniques: Construct